CAT NO	PRODUCT NAME	SIZE	PRICE
P123102	HiPure Plasmid EF Midi Kit(Vacuum)	50 preps	$423.00
P123102B	HiPure Plasmid EF Midi Kit(Spin)	50 preps	$547.00

Introduction

The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.

Details

Specifications

Features	Specifications
Main Functions	Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture
Applications	Cell transfection, animal injection, etc.
Purification method	Midi spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	25-50ml
Yield	10-500μg
Elution volume	≥0.4ml
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	250μg

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast - silica gel column purification is much faster than ion exchange
High yield - up to 250μg plasmid can be binded in one column
Low endotoxin content - the obtained plasmid can be directly used in cell transfection and animal injection

Kit Contents

Contents	P123102	P123102B
Purification Times	50 Preps	50 Preps
RNase A	30 mg	30 mg
Buffer E1	150 ml	150 ml
Buffer E2	150 ml	150 ml
Buffer E3	150 ml	150 ml
Buffer E4	150 ml	150 ml
Buffer E5	150 ml	150 ml
Buffer PW2*	50 ml	50 ml
Elution Buffer	30 ml	30 ml
MaxPure EF Midi Column	50	50
2 ml Collection Tubes	50	50
Lysate Clear Midi Syringe	50	50
Extend Tubes	50	50
50ml Centrifuge Tubes		50
Support Tubes		50

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2–8°C.

Purchase Guide

Name	CAT NO	Sample amount	Column type	Elution volume	Yield	Time per run	Centrifuge requirements
HiPure Plasmid EF Mini Kit	P1154	10-15ml	1.5ml column	≥75μl	10–70µg/15ml	≤40 minutes	small centrifuge
HiPure Plasmid EF 96 Kit	P1157	1-5ml	96 well plate	≥75μl	30µg/1ml	≤60 minutes	96 well plate centrifuge
HiPure Plasmid EF Midi Kit	P1231	30-50ml	15ml column	≥0.4ml	10-500μg/50ml	≤60 minutes	15ml centrifuge
HiPure Plasmid EF Maxi Kit	P1156	100-200ml	50ml column	≥0.8ml	100–1500µg/200ml	≤60 minutes	50ml centrifuge
HiPure Plasmid EF Mega Kit	P1116	500ml	50ml column	≥0.8ml	10mg/500ml	≤70 minutes	50ml centrifuge

Articles

【Cell Reports】Template-independent genome editing in the Pcdh15av−3j mouse, a model of human DFNB23 nonsyndromic deafness

Download

P1231 HiPure Plasmid EF Midi Kit (Spin+Vacuum) 2023.pdf Preview Down

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P1231

Principle

HiPure Plasmid EF Mini Kit

HiPure Plasmid EF Maxi Kit

HiPure Plasmid EF Mega Kit