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C12123

CAT NO PRODUCT NAME SIZE PRICE
C12123 RNase Solution 10 ml $130.00
C12124 RNase Solution 100 ml $1,040.00
C12128 DNase Free RNase Solution 10 ml $130.00
C12129 DNase Free RNase Solution 100 ml $1,040.00
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Introduction

RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphategroup attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2',3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. The highest activity is exhibited with single-stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges.

A major application for RNase A is the removal of RNA from preparations of plasmid DNA. The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well as the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.

Details

Specifications

Features Specifications
Purity 60% RNase A basis (SDS-PAGE)
Enzymatic activity >50 Kunitz units/mg protein
Optimum reaction temperature
60°C (effective active temperature is 15-70°C)
Transportation conditions
Normal temperature transportation
Preservation conditions -20-8°C, dry storage, long-term storage should be placed at -20°C.
Application 1: adding in the extraction process

1. Plasmid Extraction: add RNase A (25mg/ml) to buffer P1 with a final concentration of 100-300μg/ml.

2. DNA extraction: add RNase A (25mg/ml) to the digestion solution with a final concentration is 100-400μg/ml, mix well and place at room temperature for 10-15 minutes. when SDS/CTAB in lysate exceeds 2%, RNase activity will be significantly inhibited; Guanidine salt(>4M guanidine hydrochloride or >3M guanidine isothiocyanate) also significantly inhibited RNase A. When RNase A is added to the lysate, the RNase digestion effect can be extracted by appropriately diluting to reduce the concentration of SDS, CTAB and guanidine salt.

Application 2:

1. Remove RNA contamination from crude genomic DNA products: add DNase free RNase A (10mg/ml) to crude DNA products with a final concentration of 10-100μg/ml. After mixing, place at room temperature for 10 minutes.

2. Remove RNA contamination from plasmid DNA products: add DNase free RNase A (10mg/ml) to crude DNA products with a final concentration of 10μg/ml. After mixing, it can be directly used for sequencing at room temperature for 10 minutes.

Ordering information

Contents C12123
C12124 C12128
C12129
RNase A Solution (25mg/ml) 10 ml
100 ml

DNase Free RNase A Solution (10mg/ml)


10 ml
100 ml

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