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D3181

CAT NO PRODUCT NAME SIZE PRICE
D318102 HiPure Circulating Nucleic acid Mini Kit 50 preps $273.00
D318103
HiPure Circulating Nucleic acid Mini Kit
250 preps
$1,243.00
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Introduction

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Mini Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be fresh or frozen (provided that they have not been frozen and thawed more than once).


Details

Specifications

Features Specifications
Main Functions Isolation circulating DNA from 1ml plasma, serum, body fluids
Applications qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Serum, plasma and other cell-free fluid samples
Sample amount 1ml
Elution volume ≥30μl
Time per run ≤50 minutes
Liquid carrying volume per column 800µl
Binding yield of column 100µg


Principle

This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).

Advantages

  • High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
  • High concentration - low elution volume, ensuring high nucleic acid concentration
  • High purity - low alcohol binding method, completely removing inhibitor and protein pollution
  • High recovery - DNA can be recovered at the level of PG by silica gel column purification

Kit Contents

Contents D318102 D318103
Purification Times 50 Preps 250 Preps
Buffer ACL 50 ml 250 ml
Buffer ACB*
60 ml 300 ml
Buffer DCW1* 22 ml 88 ml
Buffer DCW2* 10 ml 50 ml
Proteinase K 120 mg 540 mg
Protease Dissolve Buffer 10 ml 30 ml
Carrier RNA 110 μg 310 μg
Nuclease Free Water
10 ml 30 ml
HiPure CFDNA Mini Columns
50 250
2 ml Collection Tubes
100 500

Storage and Stability

Proteinase K and carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage(up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers shouldbe redissolved before use. Make sure that all buffers are at room temperature when used.

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