Specifications
Features
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Specifications
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Main Functions
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Isolation up to 10µg plasmid DNA from 1.5ml bacterial culture
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Applications
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Enzyme digestion, sequencing, PCR and labeling, etc.
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Conventional plasmid, plasmid less than 30KB
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Sample amount
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1-1.5ml
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Elution volume
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≥50μl
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Time per run
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≤20 minutes
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Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
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High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
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Fast - it takes only 20 minutes to complete the isolation
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High yield - up to 20μg plasmid can be binded in one column
Kit Contents
Contents
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P181102
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P181103
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P181104
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Purification Times
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100 Preps
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500 Preps
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5000 Preps
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RNase A
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10 mg
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50 mg
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2 x 250 mg
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Buffer P1
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30 ml
|
150 ml
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2 x 750 ml
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Buffer P2
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30 ml
|
150 ml
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2 x 750 ml
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Buffer N3
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30 ml
|
150 ml
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2 x 750 ml
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Buffer PW1
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35 ml
|
180 ml
|
2 x 900 ml
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MagPure Particle NB*
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2.2 ml
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11 ml
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2 x 60 ml
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Storage and Stability
RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.
Experiment Data