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P1811

CAT NO PRODUCT NAME SIZE PRICE
P181102 MagPure Plasmid Mini Kit 100 preps $59.00
P181103 MagPure Plasmid Mini Kit 500 preps $239.00
P181104 MagPure Plasmid Mini Kit 5000 preps $2,152.00
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Introduction

The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs. The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.

Details

Specifications

Features Specifications
Main Functions Isolation up to 10µg plasmid DNA from 1.5ml bacterial culture
Applications Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type
Conventional plasmid, plasmid less than 30KB
Sample amount
1-1.5ml
Elution volume ≥50μl
Time per run 20 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
  • Fast - it takes only 20 minutes to complete the isolation
  • High yield - up to 20μg plasmid can be binded in one column

Kit Contents

Contents P181102
P181103
P181104
Purification Times 100 Preps
500 Preps 5000 Preps
RNase A 10 mg 50 mg 2 x 250 mg
Buffer P1 30 ml 150 ml 2 x 750 ml
Buffer P2
30 ml
150 ml
2 x 750 ml
Buffer N3
30 ml
150 ml
2 x 750 ml
Buffer PW1
35 ml
180 ml
2 x 900 ml
MagPure Particle NB* 2.2 ml
11 ml 2 x 60 ml

Storage and Stability

RNase A and MagPure Particle NB should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.

Experiment Data


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