Specifications
Features
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Specifications
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Main Functions
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Isolation up to 15μg endotoxin free plasmid DNA from 1-5ml bacterial culture
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Applications
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Enzyme digestion, sequencing, PCR and labeling, etc.
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Conventional plasmid, plasmid less than 30KB
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Sample amount
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1-5ml
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Elution volume
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≥50μl
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Time per run
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≤80 minutes
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Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
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High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
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Fast - it takes only 80 minutes to complete the isolation
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High yield - up to 15μg plasmid can be binded in one column
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Economic - excellent cost effectiveness performance
Kit Contents
Contents
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P181402
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P181403
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P181404
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Purification Times
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100 Preps
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500 Preps
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5000 Preps
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RNase A
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10 mg
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30 mg
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2 x 160 mg
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Buffer P1
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30 ml
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150 ml
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2 x 800 ml
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Buffer P2
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30 ml
|
150 ml
|
2 x 800 ml
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Buffer LEN3
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20 ml
|
80 ml
|
800 ml
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Buffer LN4
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90 ml
|
400 ml
|
4 x 980 ml
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MagPure Particles
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3.5 ml
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17 ml
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3 x 60 ml
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Storage and Stability
RNase A and MagPure Particles should be stored at 2–8°C upon arrival. However, short-term
storage (up to 12 weeks) at room temperature (15–25°C) does not affect its performance. The
remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at
least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when
used. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2–8°C