| CAT NO | PRODUCT NAME | SIZE | PRICE |
| D316302 | HiPure HP Plant DNA Maxi Kit | 10 preps | $224.00 |
| D316303 | HiPure HP Plant DNA Maxi Kit | 50 preps | $995.00 |
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 3g of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic, mitochondrial, and chloroplast) for reliable PCR and southern blot in less than 1 hour.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 3g plant and fungal tissue |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Various plant samples (including conventional, polysaccharides and polyphenols) |
| Sample amount |
Fresh / frozen plant samples: 2-3 g Dried plant / seed samples: 0.5-1 g |
| Elution volume | ≥500μl |
| Time per run | ≤120 minutes |
| Liquid carrying volume per column | 20ml |
| Binding yield of column | 5mg |
This product is based on silica Column purification. Plant material is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated. Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged.DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
| Contents | D316302 | D316303 |
| Purification Times | 10 Preps | 50 Preps |
|
RNase A |
20 mg |
90 mg |
|
Protease Dissolve Buffer |
1.8 ml |
10 ml |
| Buffer PAL | 180 ml | 900 ml |
| Buffer GWP | 150 ml | 800 ml |
| Buffer GW2 | 25 ml | 200 ml |
|
Buffer AE |
30 ml |
120 ml |
|
HiPure DNA Maxi Columns II |
10 |
50 |
|
50 ml Collection Tubes |
20 |
100 |
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.
| Name | CAT NO |
Fresh / frozen tissue amount |
Dried tissue amount |
Column type |
Elution volume |
Yield (muscle) |
| D3187 | 150mg | 40mg | 1.5ml column | 50 - 100μl | 3-70μg | |
| D3163 | 5g | 1g | 50ml column | 0.7 - 3ml | 75-1250μg | |
| D3164 |
100mg |
20mg |
1.5ml column |
50 - 100μl |
3-50μg |
|
| D3167 |
50mg |
15mg |
96 well plate |
75 - 150μl |
3-30μg |
