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D6352

CAT NO PRODUCT NAME SIZE PRICE
D635201 MagPure Seed DNA Kit 48 preps $119.00
D635202 MagPure Seed DNA Kit 96 preps $224.00
D635203
MagPure Seed DNA Kit
5 x 96 preps $995.00
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Introduction

MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.

Details

Specifications

Features Specifications
Main Functions Isolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed
Applications PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc.
Purification technology Magnetic beads technology
Process method Manual or automatic
Sample type Conventional economic plant samples
Sample amount 50-100mg
Elution volume ≥50μl
Time per run 60 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • Safe - require no phenol or chloroform extraction
  • Fast - several samples can be extracted in 60 minutes
  • High purity - high quality DNA, completely remove inhibitors

Kit Contents

Contents D635201
D635202 D635203
Purification Times 48 Preps
96 Preps 5 x 96 Preps
MagPure Particles
1.7 ml
4 ml
18 ml
Buffer SOL
40 ml
90 ml
500 ml
Buffer SDS 4 ml
9 ml 50 ml
Buffer MPB 40 ml
70 ml 350 ml
Buffer GW1 26 ml
53 ml 220 ml
Elution Buffer
10 ml
30 ml
120 ml

Storage and Stability

MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data




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