Specifications
Features
|
Specifications
|
Main Functions
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Recover DNA from agarose Gel by magnetic bead
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Applications
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PCR, NGS, chip analysis, etc.
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Purification technology
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Magnetic beads technology
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Process method
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Manual or automatic
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Sample type
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Agarose Gel, PCR products, enzymatic reaction solution
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Sample amount
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Agarose Gel: appropriate amount, PCR products: ≤50µl
|
Recovery
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80%
|
Elution volume
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≥30μl
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Time per run
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≤30 minutes
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Principle
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
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High recovery efficiency - up to 80% DNA recovery
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General - recover DNA from gel or enzyme-driven reaction solutions such as PCR
Kit Contents
Contents
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D500101
|
D500102
|
D500103
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Purification Times
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50 Preps
|
500 Preps
|
5000 Preps
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Buffer GDP
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30 ml
|
250 ml
|
3 x 900 ml
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MagPure Particles
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1.6 ml
|
15 ml
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3 x 50 ml
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Buffer DW1
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20 ml
|
180 ml
|
2 x 800 ml
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Elution Buffer(10mmTris, pH8.5)
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10 ml
|
60 ml
|
500 ml
|
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data