| CAT NO | PRODUCT NAME | SIZE | PRICE |
|---|---|---|---|
| R418202 | HiPure Yeast & MicroBiol RNA Kit | 50 preps | $249.00 |
| R418203 | HiPure Yeast & MicroBiol RNA Kit | 250 preps | $1,144.00 |
This kit uses phenol and bead grinding method, which is suitable for extracting high purity total RNA from yeast, bacteria, fungal mycelium or sporangium powder, and other difficult lysed microorganisms. The kit is based on silica gel column purification technology, the whole extraction process only takes about 40 minutes. The purified RNA can be directly used for RT-PCR, Northern blot, Poly-A + purification, nucleic acid protection and in vitro translation experiments, ect.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from bacteria, yeast cells |
| Applications | RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type |
Bacteria, yeast cells |
| Sample amount | Bacteria: <10^9；Yeast cells：<2 x10^7 |
| Elution volume | ≥30μl |
| Time per run |
≤40 minutes |
| Liquid carrying volume per column |
800µl |
| Binding yield of column | 100µg |
Kit Contents
| Contents | R418202 | R418203 |
| Purification Times | 50 Preps |
250 Preps |
| gDNA Filter Mini Column | 50 | 250 |
| HiPure RNA Mini Column I | 50 | 250 |
|
2ml Collection Tubes |
100 |
500 |
|
2ml Bead Tubes F |
50 |
250 |
| Buffer ATL | 30 ml | 150 ml |
| Buffer PCI | 30 ml | 150 ml |
| Buffer GDP | 20 ml | 100 ml |
|
Buffer RW2* |
20 ml |
2 x 50 ml |
|
RNase Free Water |
10 ml |
30 ml |
Storage and Stability
Buffer PCI should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
